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An Open Source Technology Platform to Manufacture Hydrogel-Based 3D Culture Models in an Automated and Standardized Fashion
JoVE 杂志
生物工程
This content is Free Access.
JoVE 杂志 生物工程
An Open Source Technology Platform to Manufacture Hydrogel-Based 3D Culture Models in an Automated and Standardized Fashion

An Open Source Technology Platform to Manufacture Hydrogel-Based 3D Culture Models in an Automated and Standardized Fashion

DOI:
10.3791/61261-v

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08:29 min

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March 31, 2022

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, , , , , ,
1Centre in Regenerative Medicine, Institute of Health and Biomedical Innovation,Queensland University of Technology, 2School of Mechanical, Medical and Process Engineering, Science and Engineering Faculty,Queensland University of Technology, 3School of Biomedical Sciences, Faculty of Health,Queensland University of Technology, 4Australian Prostate Cancer Research Centre, Institute of Health and Biomedical Innovation,Queensland University of Technology, 5Translational Research Institute,Queensland University of Technology, 6ARC ITTC in Additive Biomanufacturing, Institute of Health and Biomedical Innovation,Queensland University of Technology

Chapters

  • 00:04Introduction
  • 01:00Application Programming Interface (API) Installation
  • 01:34Protocol Code Generation
  • 03:39Protocol Execution
  • 05:12Validation and Verification
  • 06:02Results: Representative Hydrogel Characterization and Evaluation
  • 07:52Conclusion

Summary

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This protocol serves as a comprehensive tutorial for standardized and reproducible mixing of viscous materials with a novel open source automation technology. Detailed instructions are provided on the operation of a newly developed open source workstation, the usage of an open source protocol designer, and the validation and verification to identify reproducible mixtures.

Tags

Keywords: Open SourceBiomedical ResearchReproducibilityAutomationStandardizationHydrogel3D Cell CultureManufacturingAPIPipettingBiofabricationProtocol DesignGelMAAlginatePhotoinitiatorPhoto Crosslinking96-well PlateDouble Network HydrogelsAdvanced Mixing Protocol

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