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Culture of Brain Capillary Pericytes for Cytosolic Calcium Measurements and Calcium Imaging Studies
JoVE Journal
神経科学
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JoVE Journal 神経科学
Culture of Brain Capillary Pericytes for Cytosolic Calcium Measurements and Calcium Imaging Studies

Culture of Brain Capillary Pericytes for Cytosolic Calcium Measurements and Calcium Imaging Studies

DOI:

09:33 min

May 27, 2020

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  • 00:04Introduction
  • 00:41Isolation of Primary Pericytes from Bovine Brain Capillaries
  • 03:01Loading of Pericytes with Calcium Indicator Dye in a Plate Reader Setup
  • 05:18Live Imaging of Intracellular Ca2+ Levels
  • 07:11Results: Pericyte Isolation and Calcium Measurements
  • 09:02Conclusion

概要

自動翻訳

Brain capillary pericytes are essential players in the regulation of blood-brain barrier properties and blood flow. This protocol describes how brain capillary pericytes can be isolated, cultured, characterized with respect to cell type and applied for investigations of intracellular calcium signaling with fluorescent probes.

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