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Culture of Brain Capillary Pericytes for Cytosolic Calcium Measurements and Calcium Imaging Studies
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Culture of Brain Capillary Pericytes for Cytosolic Calcium Measurements and Calcium Imaging Studies
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May 27, 2020
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Brain capillary pericytes are essential players in the regulation of blood-brain barrier properties and blood flow. This protocol describes how brain capillary pericytes can be isolated, cultured, characterized with respect to cell type and applied for investigations of intracellular calcium signaling with fluorescent probes.
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Hørlyck, S., Helms, H. C. C., Brodin, B. Culture of Brain Capillary Pericytes for Cytosolic Calcium Measurements and Calcium Imaging Studies. J. Vis. Exp. (159), e61253, doi:10.3791/61253 (2020).
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