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In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
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JoVE Journal 発生生物学
In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

7,591 Views

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10:00 min

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November 28, 2019

DOI:

10.3791/60551-v

DOI:
10.3791/60551-v

•

10:00 min
November 28, 2019

•

3 Views
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1Departamento de Fisiología y Desarrollo Celular,Instituto Nacional de Perinatología, 2Biotecnología Médica y Farmacéutica CONACYT,Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), 3Departamento de Inmunobioquímica,Instituto Nacional de Perinatología, 4Instituto de Neurobiología, UNAM

概要

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This protocol describes the isolation of epithelial cells from different anatomical regions of the human amniotic membrane to determine their heterogeneity and functional properties for possible application in clinical and physiopathological models.

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Avila-González, D., García-López, G., Díaz-Martínez, N. E., Flores-Herrera, H., Molina-Hernández, A., Portillo, W., Díaz, N. F. In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane. J. Vis. Exp. (153), e60551, doi:10.3791/60551 (2019).

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