This protocol describes the isolation of epithelial cells from different anatomical regions of the human amniotic membrane to determine their heterogeneity and functional properties for possible application in clinical and physiopathological models.
Avila-González, D., García-López, G., Díaz-Martínez, N. E., Flores-Herrera, H., Molina-Hernández, A., Portillo, W., Díaz, N. F. In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane. J. Vis. Exp. (153), e60551, doi:10.3791/60551 (2019).