An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Ahmed Ali; Yasmine Abouleila; Arno Germond

doi:10.3791/60449

/

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

JoVE Journal
生化学

This content is Free Access.

JoVE Journal 生化学

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

8,915 Views

•

07:37 min

•

January 09, 2020

DOI:

10.3791/60449-v

DOI:

10.3791/60449-v

•

07:37 min

January 09, 2020

•

8 Views

Ahmed Ali^1,2, Yasmine Abouleila^1,2, Arno Germond¹

¹Biodynamics Research Center (BDR), RIKEN, ²Research Center,Misr International University

概要

This protocol presents an integrated Raman spectroscopy-mass spectrometry (MS) platform that is capable of achieving single-cell resolution. Raman spectroscopy can be used to study cellular response to drugs, while MS can be used for targeted and quantitative analysis of drug uptake and metabolism.

Explore More Videos

Raman Spectroscopy Mass Spectrometry Single-cell Analysis Drug Uptake 代謝 Cellular Heterogeneity Cancer Resistance Metabolomics Spectral Imaging Micromanipulator Cell Culture Tamoxifen DMSO Spectrophotometer Calibration Micro-chamber

Read Article

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

概要

Automatically generated

Explore More Videos