In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated <em>Caenorhabditis elegans</em> Embryo Blastomeres and Adhesive Polystyrene Beads

Christina Rou Hsu; Rain Xiong; Kenji Sugioka

doi:10.3791/60422

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In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads

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JoVE Journal 発生生物学

In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads

In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads

6,949 Views

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07:52 min

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November 26, 2019

DOI:

10.3791/60422-v

DOI:

10.3791/60422-v

•

07:52 min

November 26, 2019

•

7 Views

Christina Rou Hsu^1,2, Rain Xiong^1,2, Kenji Sugioka^1,2

¹Department of Zoology,The University of British Columbia, ²Life Sciences Institute,The University of British Columbia

概要

Tissue complexities of multicellular systems confound the identification of causal relationship between extracellular cues and individual cellular behaviors. Here, we present a method to study the direct link between contact-dependent cues and division axes using C. elegans embryo blastomeres and adhesive polystyrene beads.

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In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads

概要

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