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In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads
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In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads

In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads

6,948 Views

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07:52 min

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November 26, 2019

DOI:

10.3791/60422-v

DOI:
10.3791/60422-v

•

07:52 min
November 26, 2019

•

7 Views
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1Department of Zoology,The University of British Columbia, 2Life Sciences Institute,The University of British Columbia

Summary

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Tissue complexities of multicellular systems confound the identification of causal relationship between extracellular cues and individual cellular behaviors. Here, we present a method to study the direct link between contact-dependent cues and division axes using C. elegans embryo blastomeres and adhesive polystyrene beads.

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In Vitro ReconstitutionCell Contact PatternsCaenorhabditis ElegansEmbryo BlastomeresAdhesive Polystyrene BeadsCellular InteractionsCell-cell SignalingFluorescent LabelingMicroscopyEmbryo IsolationEDACRhodamine Red-X

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Hsu, C. R., Xiong, R., Sugioka, K. In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads. J. Vis. Exp. (153), e60422, doi:10.3791/60422 (2019).

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