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Visualization of Candida albicans in the Murine Gastrointestinal Tract Using Fluorescent In Situ Hybridization
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
Visualization of Candida albicans in the Murine Gastrointestinal Tract Using Fluorescent In Situ Hybridization

Visualization of Candida albicans in the Murine Gastrointestinal Tract Using Fluorescent In Situ Hybridization

DOI:
10.3791/60283-v

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10:08 min

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November 05, 2019

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, ,
1Department of Microbiology and Immunology,University of California, San Francisco, 2Department of Medicine, Division of Infectious Disease,University of California, San Francisco

章

  • 00:04標題
  • 01:28Gastrointestinal Colonization of Mice with C. albicans Using Oral Gavage
  • 03:30Preparation of GI Tissues for Histology
  • 05:50FISH Staining in GI Tract Tissues
  • 08:22Results: FISH-stained C. albicans In Vitro and in Different Gut Compartments
  • 09:17Conclusion

概要

自動翻訳

自動翻訳

The purpose of this protocol is to visualize Candida albicans cell shape and localization in the mammalian gastrointestinal tract.

タグ

Candida AlbicansFluorescent In Situ HybridizationGastrointestinal TractMurineVisualizationMicrobiotaInflammatory Bowel DiseaseGavageDissectionAnimal Model

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