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Visualization of Candida albicans in the Murine Gastrointestinal Tract Using Fluorescent In Situ Hybridization
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
Visualization of Candida albicans in the Murine Gastrointestinal Tract Using Fluorescent In Situ Hybridization

Visualization of Candida albicans in the Murine Gastrointestinal Tract Using Fluorescent In Situ Hybridization

DOI:
10.3791/60283-v

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10:08 min

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November 05, 2019

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, ,
1Department of Microbiology and Immunology,University of California, San Francisco, 2Department of Medicine, Division of Infectious Disease,University of California, San Francisco

Chapters

  • 00:04Title
  • 01:28Gastrointestinal Colonization of Mice with C. albicans Using Oral Gavage
  • 03:30Preparation of GI Tissues for Histology
  • 05:50FISH Staining in GI Tract Tissues
  • 08:22Results: FISH-stained C. albicans In Vitro and in Different Gut Compartments
  • 09:17Conclusion

Summary

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The purpose of this protocol is to visualize Candida albicans cell shape and localization in the mammalian gastrointestinal tract.

Tags

Candida AlbicansFluorescent In Situ HybridizationGastrointestinal TractMurineVisualizationMicrobiotaInflammatory Bowel DiseaseGavageDissectionAnimal Model

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