ジャーナル
/
生化学
/
Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
JoVE Journal
生化学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 生化学
Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

8,069 Views

•

06:52 min

•

November 01, 2019

DOI:

10.3791/60005-v

DOI:
10.3791/60005-v

•

06:52 min
November 01, 2019

•

3 Views
, , , , ,
1Department of Physics, Chemistry and Biology,Linköping University, 2Wallenberg Centre for Molecular Medicine (WCMM), 3Nucleic Acids Technologies Laboratory (NAT-lab),Linköping University

概要

Automatically generated

Altered nuclease activity has been associated with different human conditions, underlying its potential as a biomarker. The modular and easy to implement screening methodology presented in this paper allows the selection of specific nucleic acid probes for harnessing nuclease activity as a biomarker of disease.

Explore More Videos

Nuclease ActivityNucleic Acid ProbesKinetic ScreeningBiomarkerNuclease DetectionOligonucleotide LibraryBacterial CultureNuclease AssayFluorometrySalmonellaE. Coli

Read Article

Cite this Article

Balian, A., Garcia Gonzalez, J., Bastida, N., Akhtar, K. K., Borsa, B. A., Hernandez, F. J. Kinetic Screening of Nuclease Activity using Nucleic Acid Probes. J. Vis. Exp. (153), e60005, doi:10.3791/60005 (2019).

Download .ris file

Copy

Share Video

.

Copy