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Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
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Biochimica
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JoVE Journal Biochimica
Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

8,066 Views

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06:52 min

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November 01, 2019

DOI:

10.3791/60005-v

DOI:
10.3791/60005-v

•

06:52 min
November 01, 2019

•

3 Views
, , , , ,
1Department of Physics, Chemistry and Biology,Linköping University, 2Wallenberg Centre for Molecular Medicine (WCMM), 3Nucleic Acids Technologies Laboratory (NAT-lab),Linköping University

Summary

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Altered nuclease activity has been associated with different human conditions, underlying its potential as a biomarker. The modular and easy to implement screening methodology presented in this paper allows the selection of specific nucleic acid probes for harnessing nuclease activity as a biomarker of disease.

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Cite this Article

Balian, A., Garcia Gonzalez, J., Bastida, N., Akhtar, K. K., Borsa, B. A., Hernandez, F. J. Kinetic Screening of Nuclease Activity using Nucleic Acid Probes. J. Vis. Exp. (153), e60005, doi:10.3791/60005 (2019).

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