ジャーナル
/
免疫学と感染
/
Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection
JoVE Journal
免疫学と感染
This content is Free Access.
JoVE Journal 免疫学と感染
Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection

Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection

DOI:
10.3791/59819-v

•

09:11 min

•

July 16, 2019

•

, , ,
1The Kennedy Institute of Rheumatology,The University of Oxford

章

  • 00:04標題
  • 01:03Listeria monocytogenes (LM) Infection and Brefeldin A (BFA) Treatment
  • 02:02Spleen Harvesting, Fixation with Paraformaldehyde (PFA), Freezing and Sectioning
  • 04:41Immunofluorescent Staining and Imaging
  • 06:44Results: IFNγ Production
  • 08:19Conclusion

概要

自動翻訳

自動翻訳

Here, we describe a simple confocal imaging method to visualize the in situ localization of cells secreting the cytokine Interferon gamma in murine secondary lymphoid organs. This protocol can be extended for the visualization of other cytokines in diverse tissues.

タグ

In SituInterferon GammaListeria MonocytogenesMouse SpleenCytokine VisualizationMicroenvironmentLM OVA CultureIntravenous InjectionBFAEuthanizationSpleen DissectionFixative Solution

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes

Following Cell-fate in E. coli After Infection by Phage Lambda

Oral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food

Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes

Intravital Microscopy Imaging of the Liver following Leishmania Infection: An Assessment of Hepatic Hemodynamics

Assessing Bacterial Invasion of Cardiac Cells in Culture and Heart Colonization in Infected Mice Using Listeria monocytogenes

RNA Purification from Intracellularly Grown Listeria monocytogenes in Macrophage Cells

Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Listeria monocytogenes Infection of the Brain

Read Article