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Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection

Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection

DOI:
10.3791/59819-v

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09:11 min

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July 16, 2019

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, , ,
1The Kennedy Institute of Rheumatology,The University of Oxford

Capitoli

  • 00:04Titolo
  • 01:03Listeria monocytogenes (LM) Infection and Brefeldin A (BFA) Treatment
  • 02:02Spleen Harvesting, Fixation with Paraformaldehyde (PFA), Freezing and Sectioning
  • 04:41Immunofluorescent Staining and Imaging
  • 06:44Results: IFNγ Production
  • 08:19Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we describe a simple confocal imaging method to visualize the in situ localization of cells secreting the cytokine Interferon gamma in murine secondary lymphoid organs. This protocol can be extended for the visualization of other cytokines in diverse tissues.

Tags

In SituInterferon GammaListeria MonocytogenesMouse SpleenCytokine VisualizationMicroenvironmentLM OVA CultureIntravenous InjectionBFAEuthanizationSpleen DissectionFixative Solution

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