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DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
JoVE Journal
生化学
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JoVE Journal 生化学
DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

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08:04 min

October 08, 2019

DOI:

08:04 min
October 08, 2019

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概要

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Protein binding microarray (PBM) experiments combined with biochemical assays link the binding and catalytic properties of DNA primase, an enzyme that synthesizes RNA primers on template DNA. This method, designated as high-throughput primase profiling (HTPP), can be used to reveal DNA-binding patterns of a variety of enzymes.

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