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Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
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JoVE Journal 生化学
Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase

6,182 Views

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10:14 min

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November 08, 2019

DOI:

10.3791/59599-v

DOI:
10.3791/59599-v

•

10:14 min
November 08, 2019

•

6 Views
, , ,
1Cancer Biology and Genetics, College of Medicine,The Ohio State University

概要

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Protocatechuate 3,4-dioxygenase (PCD) can enzymatically remove free diatomic oxygen from an aqueous system using its substrate protocatechuic acid (PCA). This protocol describes the expression, purification, and activity analysis of this oxygen scavenging enzyme.

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Cite this Article

Messer, R. K., Lopez Jr., M. A., Senavirathne, G., Yoder, K. E. Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase. J. Vis. Exp. (153), e59599, doi:10.3791/59599 (2019).

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