Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
6,177 Views
•
10:14 min
•
November 08, 2019
DOI:
Summary
Automatically generated
Protocatechuate 3,4-dioxygenase (PCD) can enzymatically remove free diatomic oxygen from an aqueous system using its substrate protocatechuic acid (PCA). This protocol describes the expression, purification, and activity analysis of this oxygen scavenging enzyme.
Read Article
Cite this Article
Messer, R. K., Lopez Jr., M. A., Senavirathne, G., Yoder, K. E. Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase. J. Vis. Exp. (153), e59599, doi:10.3791/59599 (2019).
Copy