ジャーナル
/
遺伝学
/
Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses
JoVE Journal
遺伝学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 遺伝学
Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses

Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses

DOI:
10.3791/59428-v

•

08:10 min

•

May 02, 2019

•

, , , , , , ,
1Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary Medicine,Academy of Military Medical Sciences, 2Jilin Agricultural University, 3Jiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses

章

  • 00:04標題
  • 01:37RNA Extraction and Reverse Transcription of the Viral RNA
  • 03:08First-round PCR
  • 04:03Second-round PCR
  • 04:41Analysis of the PCR Products by Electrophoresis on Agarose Gels
  • 05:48Results: Evaluation of the Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses
  • 07:44Conclusion

概要

自動翻訳

自動翻訳

A pan-lyssavirus nested reverse transcription polymerase chain reaction has been developed to detect specifically all known lyssaviruses. Validation using rabies brain samples of different animal species showed that this method has a sensitivity and specificity equivalent to the gold standard fluorescent antibody test and could be used for routine rabies diagnosis.

タグ

LyssavirusNested RT-PCRUniversal DetectionAnimal Brain SpecimensBiological Fluid SpecimensRNA ExtractionReverse TranscriptionPCR ContaminationPositive ControlNegative ControlThermocycler

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Detection of Copy Number Alterations Using Single Cell Sequencing

A Universal Protocol for Large-scale gRNA Library Production from any DNA Source

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Methodology for Accurate Detection of Mitochondrial DNA Methylation

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) Assay for Zika Virus and Housekeeping Genes in Urine, Serum, and Mosquito Samples

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction

Read Article