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In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells
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発生生物学
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JoVE Journal 発生生物学
In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

8,917 Views

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06:21 min

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April 25, 2019

DOI:

10.3791/59359-v

DOI:
10.3791/59359-v

•

06:21 min
April 25, 2019

•

3 Views
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1Department of Life Science Frontiers, Center for iPS Cells Research and Application,Kyoto University, 2Department of Clinical Application, Center for iPS Cells Research and Application,Kyoto University

概要

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We present here a protocol for the differentiation of human induced pluripotent stem cells into each somite derivative (myotome, sclerotome, dermatome, and syndetome) in chemically defined conditions, which has applications in future disease modeling and cell-based therapies in orthopedic surgery.

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Induced Pluripotent Stem CellsIPSCSomite DerivativesMyotomeDermatomeSclerotomeSyndetomeParaxial MesodermPresomitic MesodermDelta-like Protein 1Regenerative TherapyDisease ModelingMusculoskeletal DisorderHuman Embryogenesis

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Cite this Article

Nakajima, T., Sakurai, H., Ikeya, M. In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells. J. Vis. Exp. (146), e59359, doi:10.3791/59359 (2019).

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