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Intraventricular Transplantation of Engineered Neuronal Precursors for In Vivo Neuroarchitecture Studies
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JoVE Journal 遺伝学
Intraventricular Transplantation of Engineered Neuronal Precursors for In Vivo Neuroarchitecture Studies

Intraventricular Transplantation of Engineered Neuronal Precursors for In Vivo Neuroarchitecture Studies

DOI:
10.3791/59242-v

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15:00 min

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May 11, 2019

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, ,
1Laboratory of Cerebral Cortex Development,SISSA

章

  • 00:00標題
  • 00:42The Procedure
  • 01:41Preparation of the Green Pool
  • 02:09Engineering the Green Sub-pools
  • 03:19Preparing Borosilicate Needles
  • 04:17Disinfecting the Hood and Preparing Cell Tracer
  • 04:48Assembling the Injection Tube
  • 05:20Mixing “Test” and “Control” Green Sub-Pools
  • 06:24Intraventricular Transplantation
  • 09:11Histology and Immunoflourescence
  • 10:09Morphometry
  • 12:52Results: Neuroarchitectural Analysis of Neurons Overexpressing a Neocortical Patterning Gene
  • 14:17Conclusion

概要

自動翻訳

自動翻訳

Based on in vitro lentiviral engineering of neuronal precursors, their co-transplantation into wild-type brains and paired morphometric evaluation of "test" and "control" derivatives, this method allows accurate modeling of in vivo gene control of neocortical neuron morphology in a simple and affordable way.

タグ

Intraventricular TransplantationEngineered Neuronal PrecursorsIn Vivo Neuroarchitecture StudiesGene Expression ManipulationCortical Neuronal ArchitectureTau EGFP Transgenic EmbryosLentiviral InfectionNeurosphereImmunofluorescenceMorphometric ParametersPrecursor PoolTet-Off SystemDoxycycline

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