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Cortisol Measurement in Koala (Phascolarctos cinereus) Fur
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JoVE Journal 生化学
Cortisol Measurement in Koala (Phascolarctos cinereus) Fur

Cortisol Measurement in Koala (Phascolarctos cinereus) Fur

10,476 Views

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00:09 min

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August 23, 2019

DOI:

10.3791/59216-v

DOI:
10.3791/59216-v

•

00:09 min
August 23, 2019

•

1 Views
,
1School of Science and Health,Western Sydney University

概要

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We present a protocol to determine the optimal extraction solvent to measure cortisol from koala fur. The solvents used in this protocol are methanol, ethanol and isopropanol. Determining an optimal extraction solvent will aid in reliably measuring fur to determine the impact of chronic stress on koalas.

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Cortisol MeasurementKoala FurBiomarkerChronic StressGlucocorticoid HormoneNovel MethodHPLC Grade IsopropanolAnalytical Grade MethanolAnalytical Grade IsopropanolAnalytical Grade EthanolVacuum DesiccatorBead MillExtraction TechniqueSolvent ExtractAssay Buffer

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Cite this Article

Charalambous, R., Narayan, E. Cortisol Measurement in Koala (Phascolarctos cinereus) Fur. J. Vis. Exp. (150), e59216, doi:10.3791/59216 (2019).

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