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JoVE Journal Biochemistry
Cortisol Measurement in Koala (Phascolarctos cinereus) Fur
 

Cortisol Measurement in Koala (Phascolarctos cinereus) Fur

Article DOI: 10.3791/59216-v • 09:11 min • August 23rd, 2019
August 23rd, 2019 •
1, 1
1School of Science and Health, Western Sydney University

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Resumo
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We present a protocol to determine the optimal extraction solvent to measure cortisol from koala fur. The solvents used in this protocol are methanol, ethanol and isopropanol. Determining an optimal extraction solvent will aid in reliably measuring fur to determine the impact of chronic stress on koalas.

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Cortisol Measurement Koala Fur Glucocorticoid Hormone Chronic Stress Biomarker Sensitive Method Quick Turnaround Time Quantitative Assessment Clinical Management Tool Sample Preparation Hormone Assay Laboratory Analytical Precision Balance Centrifuge Tube Isopropanol Vortex Samples Micro-precision Sieve Vacuum Desiccator
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