ジャーナル
/
生物工学
/
Designing Porous Silicon Films as Carriers of Nerve Growth Factor
JoVE Journal
生物工学
This content is Free Access.
JoVE Journal 生物工学
Designing Porous Silicon Films as Carriers of Nerve Growth Factor

Designing Porous Silicon Films as Carriers of Nerve Growth Factor

DOI:
10.3791/58982-v

•

10:12 min

•

January 25, 2019

•

, , ,
1Department of Biotechnology and Food Engineering,Technion – Israel Institute of Technology, 2Faculty of Engineering,Bar-Ilan University, 3Bar-Ilan Institute of Nanotechnologies and Advanced Materials, 4Russell Berrie Nanotechnology Institute,Technion – Israel Institute of Technology

章

  • 00:04標題
  • 00:57Oxidized Porous Silicon (PSiO2) Carrier Fabrication
  • 02:10Nerve Growth Factor (NGF) Loading and Quantification and In Vitro NGF Release from PSiO2 Carriers
  • 04:42Cell Viability and Growth in the Presence of NGF-Loaded PSiO2 Carriers
  • 06:00Cell Differentiation Analysis
  • 07:27Results: Representative Effects of NGF-Loaded PSiO2 Carriers on Neuronal Growth and Development
  • 08:58Conclusion

概要

自動翻訳

自動翻訳

Here, we present a protocol to design and fabricate nanostructured porous silicon (PSi) films as degradable carriers for the nerve growth factor (NGF). Neuronal differentiation and outgrowth of PC12 cells and mice dorsal root ganglion (DRG) neurons are characterized upon treatment with the NGF-loaded PSi carriers.

タグ

Porous SiliconNerve Growth FactorNGFCarrierBioactive ProteinReservoir ImplantFabrication ProcessElectrochemical EtchingThermal OxidationLoading SolutionELISA

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Axon Stretch Growth: The Mechanotransduction of Neuronal Growth

Soft Lithographic Functionalization and Patterning Oxide-free Silicon and Germanium

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles

Designing Silk-silk Protein Alloy Materials for Biomedical Applications

Electrospinning Growth Factor Releasing Microspheres into Fibrous Scaffolds

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery

Preparation and Characterization of Novel HDL-mimicking Nanoparticles for Nerve Growth Factor Encapsulation

Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library

Read Article