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Designing Porous Silicon Films as Carriers of Nerve Growth Factor
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Bio-ingénierie
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Journal JoVE Bio-ingénierie
Designing Porous Silicon Films as Carriers of Nerve Growth Factor

Designing Porous Silicon Films as Carriers of Nerve Growth Factor

9,541 Views

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10:12 min

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January 25, 2019

DOI:

10.3791/58982-v

DOI:
10.3791/58982-v

•

10:12 min
January 25, 2019

•

3 Views
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1Department of Biotechnology and Food Engineering,Technion – Israel Institute of Technology, 2Faculty of Engineering,Bar-Ilan University, 3Bar-Ilan Institute of Nanotechnologies and Advanced Materials, 4Russell Berrie Nanotechnology Institute,Technion – Israel Institute of Technology

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Here, we present a protocol to design and fabricate nanostructured porous silicon (PSi) films as degradable carriers for the nerve growth factor (NGF). Neuronal differentiation and outgrowth of PC12 cells and mice dorsal root ganglion (DRG) neurons are characterized upon treatment with the NGF-loaded PSi carriers.

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Rosenberg, M., Zilony, N., Shefi, O., Segal, E. Designing Porous Silicon Films as Carriers of Nerve Growth Factor. J. Vis. Exp. (143), e58982, doi:10.3791/58982 (2019).

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