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3D Scanning Technology Bridging Microcircuits and Macroscale Brain Images in 3D Novel Embedding Overlapping Protocol
JoVE Journal
神経科学
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JoVE Journal 神経科学
3D Scanning Technology Bridging Microcircuits and Macroscale Brain Images in 3D Novel Embedding Overlapping Protocol

3D Scanning Technology Bridging Microcircuits and Macroscale Brain Images in 3D Novel Embedding Overlapping Protocol

DOI:

10:14 min

May 12, 2019

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  • 00:04標題
  • 01:09Brain Surface Scan, Slicing, and Multi-electrode Array Recording
  • 05:23MRI Image Processing to Stripe Cortical Surfaces
  • 06:32Preprocessing for 3D Scan Data and Coregistration of the MRI Surface and the 3D Scan Surface
  • 08:18Results: Overlapping Accuracy Between MRIs and 3D Scans
  • 09:17Conclusion

概要

自動翻訳

This article introduces an experimental protocol using 3D scanning technology bridging two spatial scales: the macroscopic spatial scale of whole-brain anatomy imaged by MRI at >100 μm and the microscopic spatial scale of neuronal distributions using immunohistochemistry staining and a multielectrode array system and other methods (~10 μm).

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