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3D Scanning Technology Bridging Microcircuits and Macroscale Brain Images in 3D Novel Embedding Overlapping Protocol
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Capitoli
- 00:04Titolo
- 01:09Brain Surface Scan, Slicing, and Multi-electrode Array Recording
- 05:23MRI Image Processing to Stripe Cortical Surfaces
- 06:32Preprocessing for 3D Scan Data and Coregistration of the MRI Surface and the 3D Scan Surface
- 08:18Results: Overlapping Accuracy Between MRIs and 3D Scans
- 09:17Conclusion
This article introduces an experimental protocol using 3D scanning technology bridging two spatial scales: the macroscopic spatial scale of whole-brain anatomy imaged by MRI at >100 μm and the microscopic spatial scale of neuronal distributions using immunohistochemistry staining and a multielectrode array system and other methods (~10 μm).
