This article presents a bovine mammary gland biopsy using core and needle biopsy tools. Harvested tissue can be used for cell culture or to assess mammary physiology and metabolism including gene expression, protein expression, protein modifications, immunohistochemistry, and metabolite concentrations.
Daley, V. L., Dye, C., Bogers, S. H., Akers, R. M., Rodriguez, F. C., Cant, J. P., Doelman, J., Yoder, P., Kumar, K., Webster, D., Hanigan, M. D. Bovine Mammary Gland Biopsy Techniques. J. Vis. Exp. (142), e58602, doi:10.3791/58602 (2018).