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Bovine Mammary Gland Biopsy Techniques
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December 23, 2018
DOI:
1National Animal Nutrition Program, a National Research Support Project (NRSP-9), Department of Animal and Food Sciences,University of Kentucky, 2School of Performing Arts,Virginia Tech, 3Department of Large Animal Clinical Sciences, The Virginia-Maryland College of Veterinary Medicine,Virginia Tech, 4Department of Dairy Science,Virginia Tech, 5Department of Animal Biosciences,University of Guelph, 6School of Visual Arts,Virginia Tech
Summary
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This article presents a bovine mammary gland biopsy using core and needle biopsy tools. Harvested tissue can be used for cell culture or to assess mammary physiology and metabolism including gene expression, protein expression, protein modifications, immunohistochemistry, and metabolite concentrations.
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Daley, V. L., Dye, C., Bogers, S. H., Akers, R. M., Rodriguez, F. C., Cant, J. P., Doelman, J., Yoder, P., Kumar, K., Webster, D., Hanigan, M. D. Bovine Mammary Gland Biopsy Techniques. J. Vis. Exp. (142), e58602, doi:10.3791/58602 (2018).
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