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Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns)
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JoVE Journal 免疫学と感染
Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns)

Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns)

9,902 Views

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14:26 min

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April 06, 2019

DOI:

10.3791/58415-v

DOI:
10.3791/58415-v

•

14:26 min
April 06, 2019

•

11 Views
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1Univ. Bordeaux UMR INTERTRYP 177 IRD CIRAD, 2IRD UMR 177 INTERTRYP CIRAD, 3CNRS, UMR 5234, Microbiologie Fondamentale et Pathogénicité, Univ. Bordeaux, 4Centre Hospitalier Université Bordeaux

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African TrypanosomesSleeping SicknessAnimal African TrypanosomiasisTsetse FlyProtist ParasitesSerologyParasite Concentration TechniquesDEAE CelluloseAnion ExchangerCentrifugationMicroscopic ObservationDiagnosisPurified ParasitesImmunologicalBiologicalBiochemicalPharmaceuticalMolecular Biological InvestigationsPhosphate Buffered SalineGlucosePenicillinStreptomycinPhenol RedPHPotassium Phosphate

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Courtois, P., Nabos, P., Nzoumbou-Boko, R., Reix, C. E., Dauchy, F., Daulouede, S., Bringaud, F., Robinson, D. R., Vincendeau, P. Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns). J. Vis. Exp. (146), e58415, doi:10.3791/58415 (2019).

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