Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns)

Pierrette Courtois; Patricia Nabos; Romaric Nzoumbou-Boko; Christine E. Reix; Fr&#233;d&#233;ric-Antoine Dauchy; Sylvie Daulouede; Fr&#233;d&#233;ric Bringaud; Derrick  R. Robinson; Philippe Vincendeau

doi:10.3791/58415

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Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns)

JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection

Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns)

9,949 Views

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14:26 min

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April 06, 2019

DOI:

10.3791/58415-v

DOI:

10.3791/58415-v

•

14:26 min

April 06, 2019

•

11 Views

Pierrette Courtois^1,2, Patricia Nabos^1,2, Romaric Nzoumbou-Boko^1,2, Christine E. Reix³, Frédéric-Antoine Dauchy^1,2,4, Sylvie Daulouede^1,2, Frédéric Bringaud³, Derrick R. Robinson³, Philippe Vincendeau^1,2,4

¹Univ. Bordeaux UMR INTERTRYP 177 IRD CIRAD, ²IRD UMR 177 INTERTRYP CIRAD, ³CNRS, UMR 5234, Microbiologie Fondamentale et Pathogénicité, Univ. Bordeaux, ⁴Centre Hospitalier Université Bordeaux

Summary

This method of trypanosome separation from blood depends on their surface charge being less negative than mammalian blood cells. Infected blood is placed and treated on an anion-exchanger column. This method, the most fitting diagnostic for African trypanosomiasis, provides purified parasites for immunological, biological, biochemical, pharmaceutical and molecular biology investigations.

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Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns)

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