Here we present a protocol to automatically determine the locomotor performance of Drosophila at changing temperatures using a programmable temperature-controlled arena that produces fast and accurate temperature changes in time and space.
Soto-Padilla, A., Ruijsink, R., Span, M., van Rijn, H., Billeter, J. An Automated Method to Determine the Performance of Drosophila in Response to Temperature Changes in Space and Time. J. Vis. Exp. (140), e58350, doi:10.3791/58350 (2018).