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Behavior

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JoVE Journal Behavior
An Automated Method to Determine the Performance of Drosophila in Response to Temperature Changes in Space and Time
 

An Automated Method to Determine the Performance of Drosophila in Response to Temperature Changes in Space and Time

Article DOI: 10.3791/58350-v • 06:52 min • October 12th, 2018
October 12th, 2018 •
1,2, 3, 4, *4, *1
1Groningen Institute for Evolutionary Life Sciences, University of Groningen, 2Department of Cell Biology, University of Groningen, University Medical Center Groningen, 3Ruijsink Dynamic Engineering, 4Department of Psychology, University of Groningen
* These authors contributed equally

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Here we present a protocol to automatically determine the locomotor performance of Drosophila at changing temperatures using a programmable temperature-controlled arena that produces fast and accurate temperature changes in time and space.

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Automated Method Drosophila Performance Temperature Changes Space And Time Genotypes Sensory Cues Temperature Receptors Technique Fast And Precise Temperature Changes Automation Rearing Bottle Incubator Light/dark Cycles Anesthetize Virgin Flies Fly Rearing Vials Temperature-controlled Arena Temperature Phases Script Control Computer Experimental Phase Duration
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