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全猪胰腺的分离与去细胞
JoVE Journal
生物工学
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JoVE Journal 生物工学
Isolation and Decellularization of a Whole Porcine Pancreas
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全猪胰腺的分离与去细胞

9,104 Views

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09:08 min

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October 10, 2018

DOI:

10.3791/58302-v

DOI:
10.3791/58302-v

•

09:08 min
October 10, 2018

•

16 Views
, , ,
1Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy,University of Gothenburg, 2The Transplant Institute,Sahlgrenska University Hospital

概要

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整个胰腺组织工程是一个挑战, 因为它的外分泌和内分泌功能。我们展示了一个完整的猪胰的解剖方法和成功的去细胞的过程中, 清洁剂海卫 X-100, 钠脱氧和脱氧核糖核酸酶的灌注。

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Keyword Extraction:Porcine PancreasDecellularizationDissectionBlood VesselsPeristaltic PumpOrgan ChamberDetergent ContainerDetergent Collection ContainerHeparinizedFemale PigMidline IncisionSplenic LobeDuodenal LobeConnection LobeMajor Duodenal PapillaInferior EsophagusSmall IntestineColon

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Kuna, V. K., Kvarnström, N., Elebring, E., Holgersson, S. S. Isolation and Decellularization of a Whole Porcine Pancreas. J. Vis. Exp. (140), e58302, doi:10.3791/58302 (2018).

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