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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
JoVE Journal
遺伝学
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JoVE Journal 遺伝学
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

DOI:

09:51 min

May 25, 2018

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  • 00:04標題
  • 01:09RNP Preparation for Primary Cell Editing, Hematopoietic Stem/Progenitor Cell (HSPC) Preparation, and RNP Electroporation
  • 02:46C. elegans Gonad Microinjection with RNPs and Post-injection Care
  • 05:17P. hawaiensis Embryo Microinjection with RNPs and Post-injection Care
  • 08:05Results: Evaluation of Genome Editing Outcomes
  • 09:28Conclusion

概要

自動翻訳

Utilizing a preassembled Cas9 ribonucleoprotein complex (RNP) is a powerful method for precise, efficient genome editing. Here, we highlight its utility across a broad range of cells and organisms, including primary human cells and both classic and emerging model organisms.

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