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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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09:51 min
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May 25, 2018
DOI:
1Department of Molecular Cell Biology,University of California, Berkeley, 2Howard Hughes Medical Institute,University of California, Berkeley, 3Innovative Genomics Institute,University of California, Berkeley, 4Biomedical Sciences Graduate Program,University of California, San Francisco, 5Department of Microbiology and Immunology,University of California, San Francisco, 6Diabetes Center,University of California, San Francisco, 7Chan Zuckerberg Biohub, 8Department of Medicine,University of California, San Francisco, 9UCSF Helen Diller Family Comprehensive Cancer Center,University of California, San Francisco, 10Department of Integrative Biology,University of California, Berkeley
Summary
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Utilizing a preassembled Cas9 ribonucleoprotein complex (RNP) is a powerful method for precise, efficient genome editing. Here, we highlight its utility across a broad range of cells and organisms, including primary human cells and both classic and emerging model organisms.
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Farboud, B., Jarvis, E., Roth, T. L., Shin, J., Corn, J. E., Marson, A., Meyer, B. J., Patel, N. H., Hochstrasser, M. L. Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms. J. Vis. Exp. (135), e57350, doi:10.3791/57350 (2018).
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