Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca<sup Class='xref'>2+</sup>-imaging and Degranulation Assays

Volodymyr Tsvilovskyy; Alejandra Solis-Lopez; Kathrin &#214;hlenschl&#228;ger; Marc Freichel

doi:10.3791/57222

/

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca²⁺-imaging and Degranulation Assays

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免疫学と感染

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JoVE Journal 免疫学と感染

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca²⁺-imaging and Degranulation Assays

11,947 Views

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11:31 min

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July 04, 2018

DOI:

10.3791/57222-v

DOI:

10.3791/57222-v

•

11:31 min

July 04, 2018

•

7 Views

Volodymyr Tsvilovskyy¹, Alejandra Solis-Lopez¹, Kathrin Öhlenschläger¹, Marc Freichel¹

¹Institute of Pharmacology,Ruprecht-Karls Heidelberg University

概要

Here, we present a protocol to isolate and cultivate murine peritoneal mast cells. We also describe two protocols for their functional characterization: a fluorescent imaging of intracellular free Ca²⁺ concentration and a degranulation assay based on colorimetric quantification of the released β-hexosaminidase.

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Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

概要

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Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca²⁺-imaging and Degranulation Assays