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Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

11,950 Views

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11:31 min

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July 04, 2018

DOI:

10.3791/57222-v

DOI:
10.3791/57222-v

•

11:31 min
July 04, 2018

•

7 Views
, , ,
1Institute of Pharmacology,Ruprecht-Karls Heidelberg University

Summary

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Here, we present a protocol to isolate and cultivate murine peritoneal mast cells. We also describe two protocols for their functional characterization: a fluorescent imaging of intracellular free Ca2+ concentration and a degranulation assay based on colorimetric quantification of the released β-hexosaminidase.

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Tsvilovskyy, V., Solis-Lopez, A., Öhlenschläger, K., Freichel, M. Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays. J. Vis. Exp. (137), e57222, doi:10.3791/57222 (2018).

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