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小鼠肝灌注肝细胞和正弦内皮细胞的纯化
JoVE Journal
生化学
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JoVE Journal 生化学
Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion
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小鼠肝灌注肝细胞和正弦内皮细胞的纯化

48,926 Views

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08:54 min

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February 12, 2018

DOI:

10.3791/56993-v

DOI:
10.3791/56993-v

•

08:54 min
February 12, 2018

•

46 Views
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1Department of Biochemistry,University of Nebraska

概要

Automatically generated

该协议的目的是获得高生存能力和高产量的肝细胞和正弦内皮细胞。这是通过灌注肝与 IV 型胶原酶溶液通过门静脉, 其次是差异离心获得肝细胞和正弦内皮细胞。

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Cite this Article

Cabral, F., Miller, C. M., Kudrna, K. M., Hass, B. E., Daubendiek, J. G., Kellar, B. M., Harris, E. N. Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion. J. Vis. Exp. (132), e56993, doi:10.3791/56993 (2018).

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