ジャーナル
/
発生生物学
/
Zika Virus Infection of Cultured Human Fetal Brain Neural Stem Cells for Immunocytochemical Analysis
JoVE Journal
発生生物学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 発生生物学
Zika Virus Infection of Cultured Human Fetal Brain Neural Stem Cells for Immunocytochemical Analysis

Zika Virus Infection of Cultured Human Fetal Brain Neural Stem Cells for Immunocytochemical Analysis

DOI:
10.3791/56917-v

•

10:27 min

•

February 05, 2018

•

, ,
1Department of Neuroscience and Cell Biology,University of Texas Medical Branch, 2Department of Neurology,Johns Hopkins University, 3Beijing Institute for Brain Disorders,Capital Medical University

章

  • 00:04標題
  • 00:57hNSC Passage
  • 04:50Plating Adherent hNSC for Validation
  • 06:02Zika Virus Infection
  • 07:37Results: Human Fetal Brain Neural Stem Cell Infection with Zika Virus
  • 09:03Conclusion

概要

自動翻訳

自動翻訳

This article details the methods that are used to expand human fetal brain neural stem cells in culture, as well as how to differentiate them into various neuronal subtypes and astrocytes, with an emphasis on the use of neural stem cells to study Zika virus infection.

タグ

Zika VirusHuman Fetal Brain Neural Stem CellsImmunocytochemical AnalysisIn VitroNeural Stem Cell DifferentiationInnate Immune ResponseTranslational ApplicationNeurological DeficitsCell CultureCell PassageTrypsinDNaseCentrifugation

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells

Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research

Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

Read Article