Adventitious shoots can be induced on internodal segments of ipecac without phytohormone treatment. To evaluate phytohormone dynamics during adventitious shoot formation, we measured endogenous auxin and cytokinin in internodal segments by LC-MS/MS.
Koike, I., Shimomura, K., Umehara, M. Quantification of Endogenous Auxin and Cytokinin During Internode Culture of Ipecac. J. Vis. Exp. (133), e56902, doi:10.3791/56902 (2018).