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环境科学
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Quantification of Endogenous Auxin and Cytokinin During Internode Culture of Ipecac
JoVE 杂志
环境科学
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JoVE 杂志 环境科学
Quantification of Endogenous Auxin and Cytokinin During Internode Culture of Ipecac

Quantification of Endogenous Auxin and Cytokinin During Internode Culture of Ipecac

DOI:
10.3791/56902-v

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07:57 min

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March 15, 2018

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, ,
1Graduate School of Life Sciences,Toyo University

Chapters

  • 00:05Title
  • 00:51Growth Conditions to Induce Adventitious Shoots of Ipecac
  • 01:46Extraction and Purification of Phytohormones
  • 04:46LC-MS/MS Analysis of IAA and CKs
  • 05:41Results: IAA and CK Levels in Adventitious Shoots Change During Five Weeks of Culture
  • 07:12Conclusion

Summary

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Adventitious shoots can be induced on internodal segments of ipecac without phytohormone treatment. To evaluate phytohormone dynamics during adventitious shoot formation, we measured endogenous auxin and cytokinin in internodal segments by LC-MS/MS.

Tags

IpecacInternode CultureEndogenous AuxinEndogenous CytokininPhytohormone QuantificationAdventitious Shoot FormationB5 MediumZirconia BeadAcetonitrile ExtractionHPLC-MS/MS

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