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High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis (QDB)
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生化学
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JoVE Journal 生化学
High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis (QDB)

High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis (QDB)

10,589 Views

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08:10 min

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August 21, 2018

DOI:

10.3791/56885-v

DOI:
10.3791/56885-v

•

08:10 min
August 21, 2018

•

8 Views
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1Medicine and Pharmacy Research Center,Binzhou Medical University, 2Yantai Zestern Biotechnique Co. LTD, 3Precision Medicine research center,Binzhou Medical University, 4Department of Chemistry – BMC,Uppsala University

概要

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Here we demonstrate a detailed process of quantitative dot blot analysis (QDB) by determining the absolute content of a targeted protein, capping actin protein, gelsolin-like (CAPG), in three different mouse tissues. We demonstrate a high throughput, convenient, quantitative immunoblot technique for biomarker validation at the cellular and tissue level.

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Cite this Article

Qi, X., Zhang, Y., Zhang, Y., Ni, T., Zhang, W., Yang, C., Mi, J., Zhang, J., Tian, G. High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis (QDB). J. Vis. Exp. (138), e56885, doi:10.3791/56885 (2018).

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