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High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis (QDB)
Chapitres
- 00:04Titre
- 01:19Sample Preparation
- 02:22Defining the Linear Range of the QDB Analysis
- 03:19Process of QDB Analysis
- 06:11Results: Representative Result of a QDB Analysis to Determine the Absolute CAPG levels in Three Mouse Tissues
- 06:56Conclusion
Here we demonstrate a detailed process of quantitative dot blot analysis (QDB) by determining the absolute content of a targeted protein, capping actin protein, gelsolin-like (CAPG), in three different mouse tissues. We demonstrate a high throughput, convenient, quantitative immunoblot technique for biomarker validation at the cellular and tissue level.
