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January 29, 2018
DOI:
1Department of Pathology, Immunology and Experimental Medicine,University of Florida, 2Heller School for Social Policy and Management,Brandeis University, 3Department of Medicine, College of Medicine,University of Florida, 4Department of Biomedical Engineering, College of Engineering,University of Florida, 5Department of Pediatrics, College of Medicine,University of Florida
概要
Automatically generated
在这里, 我们提出了一个协议的图像人类胰腺切片三维 (3D) 使用优化的被动清除方法。这篇手稿演示了这些程序的被动光清除后, 多免疫荧光染色, 以确定关键元素的自主神经和感觉神经网络支配人类胰岛。
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Butterworth, E., Dickerson, W., Vijay, V., Weitzel, K., Cooper, J., Atkinson, E. W., Coleman, J. E., Otto, K. J., Campbell-Thompson, M. High Resolution 3D Imaging of the Human Pancreas Neuro-insular Network. J. Vis. Exp. (131), e56859, doi:10.3791/56859 (2018).
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