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Standardized Method for High-throughput Sterilization of Arabidopsis Seeds
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JoVE Journal 生物学
Standardized Method for High-throughput Sterilization of Arabidopsis Seeds

Standardized Method for High-throughput Sterilization of Arabidopsis Seeds

28,165 Views

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08:13 min

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October 17, 2017

DOI:

10.3791/56587-v

DOI:
10.3791/56587-v

•

08:13 min
October 17, 2017

•

41 Views
, , , ,
1Arabidopsis Biological Resource Center, Center for Applied Plant Sciences,The Ohio State University, 2Department of Molecular Genetics, Center for Applied Plant Sciences,The Ohio State University

概要

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The objective of this study was to determine the effects of bleach and chlorine gas sterilization on seed germination of a range of Arabidopsis genotypes grown on sterile media. Optimized sterilization protocols have been developed to prevent the growth of microbial contaminants while providing satisfactory seed survival.

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Cite this Article

Lindsey III, B. E., Rivero, L., Calhoun, C. S., Grotewold, E., Brkljacic, J. Standardized Method for High-throughput Sterilization of Arabidopsis Seeds. J. Vis. Exp. (128), e56587, doi:10.3791/56587 (2017).

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