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Standardized Method for High-throughput Sterilization of Arabidopsis Seeds
JoVE Journal
生物学
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JoVE Journal 生物学
Standardized Method for High-throughput Sterilization of Arabidopsis Seeds

Standardized Method for High-throughput Sterilization of Arabidopsis Seeds

DOI:

08:13 min

October 17, 2017

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  • 00:05標題
  • 00:41Sterilization of Arabidopsis Seeds with Bleach
  • 02:14Sterilization of Arabidopsis Seed with Chlorine Gas
  • 05:01Seed Plating and Evaluating Germination
  • 05:59Results: Effects of Bleach or Chlorine Gas Sterilization on Seed Germination
  • 07:28Conclusion

概要

自動翻訳

The objective of this study was to determine the effects of bleach and chlorine gas sterilization on seed germination of a range of Arabidopsis genotypes grown on sterile media. Optimized sterilization protocols have been developed to prevent the growth of microbial contaminants while providing satisfactory seed survival.

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