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Standardized Method for High-throughput Sterilization of Arabidopsis Seeds
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Biologia
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JoVE Journal Biologia
Standardized Method for High-throughput Sterilization of Arabidopsis Seeds

Standardized Method for High-throughput Sterilization of Arabidopsis Seeds

DOI:
10.3791/56587-v

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08:13 min

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October 17, 2017

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, , , ,
1Arabidopsis Biological Resource Center, Center for Applied Plant Sciences,The Ohio State University, 2Department of Molecular Genetics, Center for Applied Plant Sciences,The Ohio State University

Capitoli

  • 00:05Titolo
  • 00:41Sterilization of Arabidopsis Seeds with Bleach
  • 02:14Sterilization of Arabidopsis Seed with Chlorine Gas
  • 05:01Seed Plating and Evaluating Germination
  • 05:59Results: Effects of Bleach or Chlorine Gas Sterilization on Seed Germination
  • 07:28Conclusion

Summary

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The objective of this study was to determine the effects of bleach and chlorine gas sterilization on seed germination of a range of Arabidopsis genotypes grown on sterile media. Optimized sterilization protocols have been developed to prevent the growth of microbial contaminants while providing satisfactory seed survival.

Tags

Keywords: Arabidopsis SeedsSeed SterilizationHigh-throughputBleach SolutionTWEEN 20Laminar Flow HoodChlorine GasHydrochloric AcidGermination RateGenomicsProteomicsMetabolomics

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