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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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生化学
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JoVE Journal 生化学
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

11,709 Views

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10:37 min

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November 15, 2017

DOI:

10.3791/56474-v

DOI:
10.3791/56474-v

•

10:37 min
November 15, 2017

•

7 Views
, , , , , , ,
1St. Jude Proteomics Facility,St. Jude Children’s Research Hospital, 2Departments of Structural Biology and Developmental Neurobiology,St. Jude Children’s Research Hospital, 3Heilongjiang University of Chinese Medicine

概要

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We present a protocol to accurately quantitate proteins with isobaric labelling, extensive fractionation, bioinformatics tools, and quality control steps in combination with liquid chromatography interfaced to a high-resolution mass spectrometer.

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Cite this Article

High, A. A., Tan, H., Pagala, V. R., Niu, M., Cho, J., Wang, X., Bai, B., Peng, J. Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification. J. Vis. Exp. (129), e56474, doi:10.3791/56474 (2017).

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