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A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates
JoVE Journal
生物工学
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JoVE Journal 生物工学
A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates

24,638 Views

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10:33 min

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February 23, 2018

DOI:

10.3791/56412-v

DOI:
10.3791/56412-v

•

10:33 min
February 23, 2018

•

17 Views
, , , , , , ,
1Institute of Bioprocess and Biosystems Engineering,Hamburg University of Technology, 2Institute of Biotechnology, Department Medical Biotechnology,Technische Universität Berlin, 3TissUse GmbH

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A method for determination of permeability in a membrane insert system for multi-well plates and in silico parameter optimization for the calculation of diffusion coefficients using simulation are presented.

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Hsu, H., Kracht, J., Harder, L. E., Rudnik, K., Lindner, G., Schimek, K., Marx, U., Pörtner, R. A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates. J. Vis. Exp. (132), e56412, doi:10.3791/56412 (2018).

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