A method for determination of permeability in a membrane insert system for multi-well plates and in silico parameter optimization for the calculation of diffusion coefficients using simulation are presented.
Hsu, H., Kracht, J., Harder, L. E., Rudnik, K., Lindner, G., Schimek, K., Marx, U., Pörtner, R. A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates. J. Vis. Exp. (132), e56412, doi:10.3791/56412 (2018).