ジャーナル
/
神経科学
/
Whole-cell Currents Induced by Puff Application of GABA in Brain Slices
JoVE Journal
神経科学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 神経科学
Whole-cell Currents Induced by Puff Application of GABA in Brain Slices

Whole-cell Currents Induced by Puff Application of GABA in Brain Slices

DOI:
10.3791/56387-v

•

07:32 min

•

October 12, 2017

•

, , ,
1School of Psychology,South China Normal University, 2School of Life Sciences,South China Normal University, 3Brain Science Institute,South China Normal University, 4Center for Studies of Psychological Application,South China Normal University, 5Guangdong Key Laboratory of Mental Health and Cognitive Science,South China Normal University

章

  • 00:05標題
  • 00:54Preparation of Prefrontal Cortex Slice
  • 02:57Whole-cell Patch Recording
  • 05:57Results: Dose and Puff-duration Response Curves
  • 06:30Conclusion

概要

自動翻訳

自動翻訳

We describe the puff technique, by which pharmacological reagents can be administered during whole-cell patch-clamp recording, and highlight various aspects of the features that are crucial for its success.

タグ

Whole-cell CurrentsGABABrain SlicesPuff Application電気生理学Pharmacological AdministrationAcute Prefrontal Cortex SliceVibratomeACSFRecording ElectrodesPuff MicropipettesSodium Channel Blocker

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

GABA-activated Single-channel and Tonic Currents in Rat Brain Slices

Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices

Paired Whole Cell Recordings in Organotypic Hippocampal Slices

Ex Vivo Optogenetic Dissection of Fear Circuits in Brain Slices

Whole-cell Patch-clamp Recordings in Brain Slices

Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices

Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays

Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons

Assessment of Long-term Depression Induction in Adult Cerebellar Slices

Read Article